Journal: Cancers

Article Title: Protein Disulphide Isomerase A1 Is Involved in the Regulation of Breast Cancer Cell Adhesion and Transmigration via Lung Microvascular Endothelial Cells

doi: 10.3390/cancers12102850

Figure Lengend Snippet: Effects of PDIA1 inhibition on wound-healing and migration of breast cancer cells and endothelial cells. ECIS Wound-Healing Assay of hLMVEC, MCF-7 and MDA-MB-231 cells and breast cancer sublines with silencing of PDIA1 (shN, shPDIA1-1 and shPDIA1-3). Real time tracings in hLMVECs ( A ), MCF-7 ( C ) and MDA-MB-231 ( E ) cell lines after addition of bepristat 2a at various concentrations (1, 10, 30 or 50 µM) and after PDIA1 silencing in MCF-7 ( G ) and MDA-MB-231 ( I ) cell lines. Area under the curve boxplots represent AUC quantitation of changes in migration rate of bepristat 2a-treated hLMVECs ( B ), MCF-7 ( D ) and MDA-MB-231 ( F ) cell lines versus non-treated controls as well as MCF-7 ( H ) or MDA-MB-231 ( J ) sublines transduced against PDIA1 (shPDIA1-1, shPDIA1-3) or wild type cells regarding to negative sequence (shN). The line graphs and AUC boxplots represent mean ± SD of three independent experiments. Statistical analysis was calculated using parametric one-way ANOVA followed by Dunnett’s multiple comparisons test (* p = 0.05, ** p = 0.01, *** p = 0.001).

Article Snippet: Human lung microvascular endothelial cell line (hLMVECs) was obtained from Cell Applications (San Diego, CA, USA).

Techniques: Inhibition, Migration, Wound Healing Assay, Quantitation Assay, Sequencing

Journal: Cancers

Article Title: Protein Disulphide Isomerase A1 Is Involved in the Regulation of Breast Cancer Cell Adhesion and Transmigration via Lung Microvascular Endothelial Cells

doi: 10.3390/cancers12102850

Figure Lengend Snippet: Effects of exogenous PDIA1 and PDIA3 on adhesive interaction between breast cancer cells and different substrates. Effect of exogenous proteins PDIA1 and PDIA3 on adhesion of MCF-7 ( A – F ) and MDA-MB-231 ( G – L ) cells to collagen type I, fibronectin and lung microvascular hLMVEC cells, respectively. Data represent mean ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test (* p = 0.05, ** p = 0.01, *** p = 0.001).

Article Snippet: Human lung microvascular endothelial cell line (hLMVECs) was obtained from Cell Applications (San Diego, CA, USA).

Techniques: Adhesive

Journal: Journal of Biomedical Optics

Article Title: Functionalized erythrocyte-derived optical nanoparticles to target ephrin-B2 ligands

doi: 10.1117/1.JBO.24.8.085002

Figure Lengend Snippet: Ephrin-B2 Western blot expression. Western blot showing the expression of ephrin-B2 in hDMVECs infected with an ephrin-B2 lentivirus compared to the ephrin-B2 expression level of hDMVECs not infected with the ephrin-B2 lentivirus. Beta-actin levels are shown as the internal loading control.

Article Snippet: Human dermal microvascular endothelial cells (hDMVECs) were cultured in endothelial cell basal medium (ECBM) with growth supplement (Cell Applications, Inc.) as previously described.

Techniques: Western Blot, Expressing, Infection, Control

Journal: Journal of Biomedical Optics

Article Title: Functionalized erythrocyte-derived optical nanoparticles to target ephrin-B2 ligands

doi: 10.1117/1.JBO.24.8.085002

Figure Lengend Snippet: Cellular fluorescence of control and ephrin-B2 hDMVECs incubated with F-NETs at various ρ * values. (a) Fluorescent images of control and ephbin-B2 hDMVECs after 40 min of incubation at 4°C with F-NETs solutions. All images are falsely colored with the blue and red corresponding to DAPI and ICG NIR emission from the NETs, respectively. Scale bars are 30 μ m . (b) Averaged fluorescence intensity ( I ¯ ) [see Eq. (3)] as a function of ρ * . Cells from 3 to 4 images were analyzed, resulting in 16 to 41 measurements for each combination of nanoparticles and cells. Statistical significance of p < 0.001 is denoted by ***. Only statistically significant populations with the same ρ * value of F-NETs are indicated. (c) Sigmoidal fit to the I ¯ values of the ephrin-B2 hDMVECs versus the ρ * value of F-NETs. Error bars in (b) and (c) represent SDs.

Article Snippet: Human dermal microvascular endothelial cells (hDMVECs) were cultured in endothelial cell basal medium (ECBM) with growth supplement (Cell Applications, Inc.) as previously described.

Techniques: Fluorescence, Control, Incubation